Biophysical Characterization of Proteins in Developing Biopharmaceuticals 2nd Edition Damian J. Houde
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Biophysical Characterization of Proteins in Developing Biopharmaceuticals 2nd Edition Damian J. Houde- Ebook Instant Download/Delivery ISBN(s): 9780444641748,0444641742
Product details:
- ISBN 10:0444641742
- ISBN 13:9780444641748
- Author: Damian J. Houde, Steven A. Berkowitz
Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today’s industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals.
Table contents:
Chapter 1. The complexity of protein structure and the challenges it poses in developing biopharmaceuticals
Chapter 2. Biophysical characterization and its role in the biopharmaceutical industry
Chapter 3. Biopharmaceutical industry’s biophysical toolbox
Chapter 4. An introduction and hierarchical organization of the biophysical tool in section II
Chapter 5. UV-absorbance, fluorescence and FT-IR spectroscopy in biopharmaceutical development
Chapter 6. Biopharmaceutical applications of protein characterisation by circular dichroism spectroscopy
Chapter 7. Size-exclusion chromatography (SEC) in biopharmaceutical process development
Chapter 8. Scattering techniques for the characterization of biopharmaceuticals
Chapter 9. Characterizing biopharmaceuticals using analytical ultracentrifugation
Chapter 10. Submicrometer, micrometer and visible particle analysis in biopharmaceutical research and development
Chapter 11. Differential scanning calorimetry in the biopharmaceutical sciences
Chapter 12. Biophysical mass spectrometry for biopharmaceutical process development: focus on hydrogen/deuterium exchange
Chapter 13. One- and two-dimensional NMR techniques
Chapter 14. Chromatography (other than size-exclusion chromatography) and electrophoresis
Chapter 15. Challenges with the biophysical characterization of complex (multi-chain assembly, chemically modified, big and high concentration) biopharmaceuticals
Chapter 16. The rigor of statistical analysis in assessing biophysical data: A lifecycle approach
Chapter 17. Developability in biophysical characterization
Chapter 18. Technical decision making using biophysical data
Chapter 19. Biophysical characterization: an integral part of the “totality of the evidence” concept
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