Chaperones: Methods and Protocols 2nd Edition John M. Walker

Chaperones: Methods and Protocols 2nd Edition John M. Walker – Ebook Instant Download/Delivery ISBN(s): 978-1071633410,1071633414

Product details:

• ISBN-10 ‏ : ‎ 1071633414
• ISBN-13 ‏ : ‎ 978-1071633410
• Author: John M. Walker

This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; the role of heat shock protein 90 (Hsp90) in gene expression through chromosome-immunoprecipitation; features of chaperone function and biology; the emerging role of the extracellular HSPs; and the use of chaperones as biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of contents:

Chapter 1: Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
Chapter 2: Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized
Chapter 3: Role of Heat Shock Factors in Stress-Induced Transcription: An Update
Chapter 4: A Workflow Guide to RNA-Seq Analysis of Chaperone Function and Beyond
Chapter 5: Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90)
Chapter 6: Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting
Chapter 7: Targeted Replacement of HSF1 Phosphorylation Sites at S303/S307
Chapter 8: Bimolecular Fluorescence Complementation Assay to Evaluate HSP90-Client
Chapter 9: Complementation Assays for Co-chaperone Function
Chapter 10: Optimized Microscale Protein Aggregation Suppression Assay: A Method for
Chapter 11: Detecting Posttranslational Modifications of Hsp90 Isoforms
Chapter 12: Multiple Targeting of HSP Isoforms to Challenge Isoform Specificity and
Chapter 13: Using a Modified Proximity Ligation Protocol to Study the Interaction Between
Chapter 14: Use of Native-PAGE for the Identification of Epichaperomes in Cell Lines
Chapter 15: Molecular Chaperone Receptors: An Update
Chapter 16: A Novel Heat Shock Protein 70-Based Vaccine Prepared from DC Tumor
Chapter 17: Methods to Assess the Impact of Hsp90 Chaperone Function on Extracellular
Chapter 18: Proteomic Profiling of the Extracellular Vesicle Chaperone in Cancer
Chapter 19: A Modified Differential Centrifugation Protocol for Isolation and Quantitation of
Chapter 20: Immunohistochemistry of Human Hsp60 in Health and Disease: Recent
Chapter 21: Multiplex Immunostaining Method to Distinguish HSP Isoforms in Cancer Tissue Specimens
Chapter 22: Large-Scale Databases and Portals on Cancer Genome to Analyze Chaperone
Chapter 23: Immunohistochemical, Flow Cytometric, and ELISA-Based Analyses of Intracellular, Membrane-Expressed, and Extracell…

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