(Ebook PDF) Exosomes and Microvesicles Methods and Protocols (Methods in Molecular Biology 1545) 1st edition by Andrew F Hill 1493967266 978-1493967261 full chapters

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Product details:

ISBN-10 : 1493967266

ISBN-13 : 978-1493967261

Author: Andrew F Hill

This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Exosomes and Microvesicles: Methods and Protocols (Methods in Molecular Biology, 1545) 1st Table of contents:

Methods to analyze EVs / Bernd Giebel and Clemens Helmbrecht

Tunable resistive pulse sensing for the characterization of extracellular vesicles

Immuno-characterization of exosomes using nanoparticle tracking analysis

Imaging and quantification of extracellular vesicles by transmission electron microscopy

Quantitative analysis of exosomal miRNA via qPCR and digital PCR

Small RNA library construction for exosomal RNA from biological samples for the Ion Torrent PGM and Ion S5TM system

Protocol for isolation and proteomic characterization of distinct extracellular vesicle subtypes by sequential centrifugal ultrafiltration

Multiplexed phenotyping of small extracellular vesicles using protein microarray (EV Array)

Purification and analysis of exosomes released by mature cortical neurons following synaptic activation

Method for isolation of extracellular vesicles and characterization of exosomes from brain extracellular space

Isolation of exosomes and microvesicles from cell culture systems to study prion transmission

Isolation of platelet-derived extracellular vesicles

Bioinformatics tools for extracellular vesicles research

Preparation and isolation of siRNA-loaded extracellular vesicles

Interaction of extracellular vesicles with endothelial cells under physiological flow conditions

Flow cytometric analysis of extracellular vesicles

 

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