Flower Development: Methods and Protocols 2nd Edition John 1071632981 9781071632987

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Product details:

• ISBN-10 ‏ : ‎ 1071632981
• ISBN-13 ‏ : ‎ 978-1071632987
• Author: John M. Walker

This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Table of contents:

1. Flower Development in Arabidopsis
2. Flower Development in the Solanaceae
3. The ABC of Flower Development in Monocots: The Model of Rice Spikelet
4. Model Species to Investigate the Origin of Flowers
5. Hormones and Flower Development in Arabidopsis
6. Genetic and Phenotypic Analyses
7. Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors
8. Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development
9. Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice
10. Isolation of Meiocytes and Cytological Analyses of Male Meiotic Chromosomes in Soybean,
11. Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis
12. Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis
13. Experimental Systems
14. Floral Induction Systems for the Study of Arabidopsis Flower Developmen
15. Protoplasting and Fluorescence-Activated Cell Sorting of the Shoot Apical Meristem Cell Types
16. Protoplast Isolation for Plant Single-Cell RNA-seq
17. Plant Nuclei Isolation for Single-Nucleus RNA Sequencing
18. Isolation of Nuclei Tagged in Specific Cell Types (INTACT) in Arabidopsis

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