(Ebook PDF) Gene Cloning and DNA Analysis 8th Edition by Brown 1119640679 9781119640677 full chapters

Gene Cloning and DNA Analysis 8th Edition by T. A. Brown – Ebook PDF Instant Download/DeliveryISBN:   1119640679, 9781119640677 

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Product details:

ISBN-10 :  1119640679 

ISBN-13 :  9781119640677

Author : T. A. Brown 

Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within.  

Gene Cloning and DNA Analysis 8th Table of contents:

PART I: The Basic Principles of Gene Cloning and DNA Analysis
Chapter 1: Why Gene Cloning and DNA Analysis are Important
1.1 The early development of genetics
1.2 The advent of gene cloning and the polymerase chain reaction
1.3 What is gene cloning?
1.4 What is PCR?
1.5 Why gene cloning and PCR are so important
1.6 How to find your way through this book
FURTHER READING
Chapter 2: Vectors for Gene Cloning: Plasmids and Bacteriophages
2.1 Plasmids
2.2 Bacteriophages
FURTHER READING
Chapter 3: Purification of DNA from Living Cells
3.1 Preparation of total cell DNA
3.2 Preparation of plasmid DNA
3.3 Preparation of bacteriophage DNA
FURTHER READING
Chapter 4: Manipulation of Purified DNA
4.1 The range of DNA manipulative enzymes
4.2 Enzymes for cutting DNA – restriction endonucleases
4.3 Ligation – joining DNA molecules together
FURTHER READING
Chapter 5: Introduction of DNA into Living Cells
5.1 Transformation – the uptake of DNA by bacterial cells
5.2 Identification of recombinants
5.3 Introduction of phage DNA into bacterial cells
5.4 Introduction of DNA into non‐bacterial cells
FURTHER READING
Chapter 6: Cloning Vectors for E. coli
6.1 Cloning vectors based on E. coli plasmids
6.2 Cloning vectors based on λ bacteriophage
6.3 Cloning vectors for synthesis of single‐stranded DNA
6.4 Vectors for other bacteria
FURTHER READING
Chapter 7: Cloning Vectors for Eukaryotes
7.1 Vectors for yeast and other fungi
7.2 Cloning vectors for higher plants
7.3 Cloning vectors for animals
FURTHER READING
Chapter 8: How to Obtain a Clone of a Specific Gene
8.1 The problem of selection
8.2 Direct selection
8.3 Identification of a clone from a gene library
8.4 Methods for clone identification
FURTHER READING
Chapter 9: The Polymerase Chain Reaction
9.1 PCR in outline
9.2 PCR in more detail
9.3 After the PCR: studying PCR products
9.4 Real‐time PCR
FURTHER READING
PART II: The Applications of Gene Cloning and DNA Analysis in Research
Chapter 10: Sequencing Genes and Genomes
10.1 Chain‐termination DNA sequencing
10.2 Next‐generation sequencing
10.3 How to sequence a genome
FURTHER READING
Chapter 11: Studying Gene Expression and Function
11.1 Studying the RNA transcript of a gene
11.2 Studying the regulation of gene expression
11.3 Identifying and studying the translation product of a cloned gene
FURTHER READING
Chapter 12: Studying Genomes
12.1 Locating the genes in a genome sequence
12.2 Determining the function of an unknown gene
12.3 Genome browsers
FURTHER READING
Chapter 13: Studying Transcriptomes and Proteomes
13.1 Studying transcriptomes
13.2 Studying proteomes
FURTHER READING
PART III: The Applications of Gene Cloning and DNA Analysis in Biotechnology
Chapter 14: Production of Protein from Cloned Genes
14.1 Special vectors for expression of foreign genes in E. coli
14.2 General problems with the production of recombinant protein in E. coli
14.3 Production of recombinant protein by eukaryotic cells
FURTHER READING
Chapter 15: Gene Cloning and DNA Analysis in Medicine
15.1 Production of recombinant pharmaceuticals
15.2 Identification of genes responsible for human diseases
15.3 Gene therapy
FURTHER READING
Chapter 16: Gene Cloning and DNA Analysis in Agriculture
16.1 The gene addition approach to plant genetic engineering
16.2 Gene subtraction
16.3 Gene editing with a programmable nuclease
16.4 Are GM plants harmful to human health and the environment?
FURTHER READING
Chapter 17: Gene Cloning and DNA Analysis in Forensic Science and Archaeology
17.1 DNA analysis in the identification of crime suspects
17.2 Studying kinship by DNA profiling
17.3 Sex identification by DNA analysis
17.4 Archaeogenetics – using DNA to study human prehistory

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