Larone’s Medically Important Fungi: A Guide to Identification 7th Edition by Lars F. Westblade, Eileen M. Burd, Shawn R. Lockhart, Gary W. Procop – Ebook PDF Instant Download/DeliveryISBN: 1683674421, 9781683674429
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ISBN-10 : 1683674421
ISBN-13 : 9781683674429
Author : Lars F. Westblade, Eileen M. Burd, Shawn R. Lockhart, Gary W. Procop
Larone’s MEDICALLY IMPORTANT FUNGI
The definitive guide for identifying fungi from clinical specimens
With a new team of authors, Larone’s Medically Important Fungi, Seventh Edition, continues the longstanding tradition of high-quality content to expand your knowledge and support your work in clinical mycology by:
Larone’s Medically Important Fungi: A Guide to Identification 7th Table of contents:
How To Use the Guide
Use of Reference Laboratories and Regulations for Transport
Safety Precautions
Taxonomy and Nomenclature
PART I: Direct Microscopic Examination of Clinical SpecimensDirect Microscopic Examination of Clinical Specimens
Introduction
Histological Terminology
Tissue Reactions to Fungal Infection
Stains
Guide to Interpretation of Direct Microscopic Examination
Detailed Descriptions
Actinomycosis
Mycetoma (Actinomycotic or Eumycotic)
Nocardiosis
Mucormycosis (Zygomycosis)
Aspergillosis
Miscellaneous Hyalohyphomycoses (Other than Aspergillosis)
Dermatophytosis (Tinea, Ringworm)
Tinea versicolor
Tinea nigra
Phaeohyphomycosis
Chromoblastomycosis
Sporotrichosis
Histoplasmosis
Emergomycosis
Talaromycosis (Penicilliosis)
Blastomycosis
Paracoccidioidomycosis
Lobomycosis
Candidiasis
Trichosporonosis
Cryptococcosis
Pneumocystosis
Protothecosis
Coccidioidomycosis
Rhinosporidiosis
Adiaspiromycosis
PART II: Identification of Fungi in Culture
Guide to Identification of Fungi in Culture
FILAMENTOUS BACTERIA
MONOMORPHIC YEASTS AND YEASTLIKE ORGANISMS
THERMALLY DIMORPHIC AND/OR ENDEMIC FUNGI
THERMALLY MONOMORPHIC MOULDS
Detailed Descriptions
Filamentous Bacteria
Yeasts and Yeastlike Organisms
Thermally Dimorphic and/or Endemic Fungi
Thermally Monomorphic Moulds
PART III: Basics of Molecular Methods for Fungal Identification
Introduction
Fungal Targets
Classic Molecular Identification Methods
Polymerase Chain Reaction
Non‐Sequencing‐Based Identification Methods
MALDI‐TOF Mass Spectrometry
Signal Amplification Methods
Nucleic Acid Amplification Methods
Sequencing‐Based Identification Methods
Sanger Sequencing
Massive Parallel or Next‐Generation Sequencing
Applications of DNA Sequencing
Accurate Molecular Identification
Phylogenetic Analysis
Organism Typing
Detection of Genetic Determinants of Resistance
PART IV: Laboratory Technique
Laboratory Procedures
Collection and Preparation of Specimens
Methods for Direct Microscopic Examination of Specimens
Primary Isolation
Macroscopic Examination of Cultures
Microscopic Examination of Growth
Procedure for Identification of Yeasts
Isolation of Yeast When Mixed with Bacteria
Germ Tube Test for the Presumptive Identification of Candida albicans
Rapid Enzyme Tests for the Presumptive Identification of Candida albicans
Caffeic Acid Disk Test
Olive Oil Disks for Culturing Malassezia spp.
Conversion of Thermally Dimorphic Fungi in Culture
Sporulation Inducement Method for Apophysomyces and Saksenaea
In vitro Hair Perforation Test (for Differentiation of Trichophyton mentagrophytes and Trichophyton rubrum)
Temperature Tolerance Testing
Maintenance of Stock Fungal Cultures
Controlling Mites
Staining Methods
Acid‐Fast Modified Kinyoun Stain for Nocardia spp.
Acid‐Fast Stain for Ascospores
Ascospore Stain
Calcofluor White Stain
Giemsa Stain
Gomori Methenamine Silver (GMS) Stain
Gram Stain (Hucker Modification)
Lactophenol Cotton Blue
Lactophenol Cotton Blue with Polyvinyl Alcohol (PVA) (Huber’s PVA Mounting Medium, Modified)
Rehydration of Paraffin‐Embedded Tissue (Deparaffination)
Media
Ascospore Media
Assimilation Media (for Yeasts)
Birdseed Agar (Niger Seed Agar; Staib Agar)
Brain Heart Infusion (BHI) Agar
Buffered Charcoal‐Yeast Extract (BCYE) Agar
Canavanine Glycine Bromothymol Blue (CGB) Agar
Casein Agar
CHROMagar Candida Medium
CHROMagar Candida Plus Medium
CHROMID Candida Agar
Chromogenic Candida Agar (Brilliance Candida Agar)
Cornmeal Agar
Dermatophyte Test Medium (DTM)
Esculin Agar
Fermentation Broth for Yeasts
Inhibitory Mould Agar (IMA)
Leeming‐Notman Agar (Modified)
Lysozyme Medium
Mycosel Agar
Potato Dextrose Agar and Potato Flake Agar
Rapid Assimilation of Trehalose (RAT) Broth
Sabouraud Brain Heart Infusion Agar (SABHI Agar)
Sabouraud Dextrose Agar (SDA)
Sabouraud Dextrose Agar with 15% NaCl
Sabouraud Dextrose Broth
Starch Hydrolysis Agar
Trichophyton Agars
Tyrosine Agar
Urea Agar
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