(Ebook PDF) Lipid Modification by Enzymes and Engineered Microbes 1st edition by Uwe T Bornscheuer 0128131683 9780128131688 full chapters

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ISBN-10 :  0128131683 

ISBN-13 :  9780128131688

Author: Uwe T Bornscheuer 

Lipid Modification by Enzymes and Engineered Microbes covers the state-of-the art use of enzymes as natural biocatalysts to modify oils, also presenting how microorganisms, such as yeast, can be designed. In the past ten years, the field has made enormous progress, not only with respect to the tools developed for the development of designer enzymes, but also in the metabolic engineering of microbes, the discovery of novel enzyme activities, and in reaction engineering/process development. For the first time, these advances are covered in a single-volume that is edited by leading enzymatic scientist Uwe Borchscheuer and authored by an international team of experts.

Lipid Modification by Enzymes and Engineered Microbes 1st Table of contents:

Chapter 1: Enzymes in Lipid Modification: An Overview
Abstract
Introduction
Practical considerations for the use of biocatalysts
Lipases
Conclusions
Chapter 2: Protein engineering of enzymes involved in lipid modification
Abstract
Introduction
Computational Tools for Enzyme Design and Engineering
Directed Evolution
Rational Design
Semirational Design
Chimeric Enzymes
De Novo Design
Conclusions
Acknowledgements
Chapter 3: Lipases/Acyltransferases for Lipid Modification in Aqueous Media
Abstract
Introduction
Acyltransfer Reactions in Aqueous Media
Phylogeny and Structural Features of Lipases/Acyltransferases
Conclusions
Chapter 4: Enzymatic Modification of Phospholipids by Phospholipase D
Abstract
Introduction
Reaction Engineering with PLD as a Biocatalyst
Structure–Function Relationship in PLDs
Concluding Remarks
Chapter 5: Enzymatic Decarboxylation as a Tool for the Enzymatic Defunctionalization of Hydrophobic Bio-based Organic Acids
Abstract
Introduction
Oxidative Decarboxylation of Fatty Acids
Decarbonylation of Fatty Acids Bound to ACP and ACYL Coenzyme A
Release of Long-Chain Olefins from Type I PKSs
Decarboxylation of Hydroxy Cinnamic Acids and Cinnamic Acids
Olefin Production in Engineered Microbes
Conclusions
Chapter 6: Hydratase, Dehydrogenase, Isomerase, and Enone Reductase Involved in Fatty Acid Saturation Metabolism
Summary
Introduction
PUFA Saturation by Gut Lactic Acid Bacteria Affecting Host Lipid Composition
Characteristics of the Enzymes Involved in PUFA Saturation Metabolism
Hydroxy Fatty Acid Dehydrogenase
Enone Reductase
Hydroxy Fatty Acid Production
Conclusions
Acknowledgements
Chapter 7: Regiospecific Conversion of Lipids and Fatty Acids through Enzymatic Cascade Reactions
Abstract
Biotransformation Pathways for the Regiospecific Conversion of Lipids and Fatty Acids
Engineering of Enzymes and Whole-Cell Biocatalysts
Bioprocess Engineering
Conclusions
Acknowledgment
Chapter 8: Enzymatic Gum Treatment
Abstract
Historic Overview of Degumming Processes
Chemistry and Physical Chemistry of Phospholipids
Phospholipase Enzymes
Enzymatic Gum Treatment Processes
Discussion
Chapter 9: Applications of Structured Lipids in Selected Food Market Segments and their Evolving Consumer Demands
Abstract
Structured Lipids in Commercial Applications
Methodology for the Synthesis of SLs
Considerations on Reaction Conditions for the Enzymatic Synthesis of SLs
SLs in Selected Applications
Omega-3 Fatty Acid-Enriched Oils for Improved Nutrition and Health
HMF Analogs for use in Infant Formula
SLs in Margarine and Shortening
Conclusions
Chapter 10: Biodiesel Production Using Lipases
Abstract
Introduction
Current Chemical Technologies for Biodiesel Production
Basic Reactions in Enzymatic Biodiesel Production
Kinetics of Enzymatic Production of Biodiesel
Lipases for Enzymatic Biodiesel Production
Reaction Systems
Industrial Processes
Conclusions
Chapter 11: Microbial and Enzymatic Synthesis of Polymers
Summary
MICROBIAL POLYMERIZATIONS OF FATTY ACIDS TO PRODUCE POLY(HYDROXYALKANOATES) PHAs: BACKGROUND
PHA PRODUCTION FROM PLANT OILS AND CORRESPONDING FAs
PHA PRODUCTION FROM WASTE OR COPRODUCT DERIVED FAs
METABOLIC ENGINEERING TO CONVERT FAs TO mcl-PHA HOMOPOLYMERS
IN VITRO OR CELL-FREE ENZYME CATALYZED POLYMERIZATIONS OF FA AND THEIR DERIVATIVES
Cutinase-Catalyzed Condensation Polymerizations
Conclusions
Chapter 12: Conventional and Oleaginous Yeasts as Platforms for Lipid Modification and Production
Abstract
Introduction
Lipid Production with Oleaginous Yeast
Tools for the Optimization of Lipid Production by Yeasts
Conclusion
Chapter 13: Enzymatic Synthesis of Glycolipid Surfactants
Abstract
Introduction
Synthesis of Glycolipid Surfactants by Enzymatic Modification of Microbial Glycolipid Surfactants
De Novo Synthesis of Glycolipid Surfactants
Conclusions
Chapter 14: Sophorolipid Modification: The Power of Yeasts and Enzymes
Abstract
Introduction
Controlling The Fatty Acid Tail by Applying Special Substrates
Yeast Genetic Engineering For Modified Sophorolipids
Enzymatic Modification of Sophorolipids
Concluding Remarks
Chapter 15: Upscaling of Enzymatic Processes
Abstract
Introduction
Structured Lipids
Bulk Fats for Margarine and Shortenings
Enzymatic Degumming
Production of Triacylglycerides with High Levels of Omega-3 Fatty Acids from Fish Oil
Biodiesel from Low-Quality Feedstocks
Production of Oleochemicals and Esters for Cosmetic Applications
Production of Fatty Acids
Environmental Benefits of Enzyme Processing

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