Molecular Biotechnology 6th Edition by Bernard Glick, Cheryl Patten 1683673668 9781683673668

Molecular Biotechnology 6th Edition by Bernard R. Glick, Cheryl L. Patten – Ebook PDF Instant Download/DeliveryISBN:  1683673668, 9781683673668 

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ISBN-10 ‏ : ‎1683673668 

ISBN-13 ‏ : ‎9781683673668

Author:   Bernard R. Glick, Cheryl L. Patten 

An authoritative introduction to the fast-changing world of molecular biotechnology

In continuous publication since 1994 and now in its sixth edition, Molecular Biotechnology: Principles and Applications of Recombinant DNA has been effective in introducing this complex field to students for more than 25 years. This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances.

Molecular Biotechnology 6th Table of contents:

1 The Development of Molecular Biotechnology
Emergence of Molecular Biotechnology
Recombinant DNA Technology
Commercialization of Molecular Biotechnology
Concerns and Consequences
Summary
References
Review Questions
2 Fundamental Technologies
Molecular Cloning
Genome Engineering Using CRISPR Technology
Polymerase Chain Reaction
Chemical Synthesis of Genes
DNA Sequencing Technologies
Sequencing Whole Genomes
Genomics
Summary
References
Review Questions
3 Production of Recombinant Proteins
Protein Production in Prokaryotic Hosts
Heterologous Protein Production in Eukaryotic Cells
Protein Engineering
Summary
References
Review Questions
4 Molecular Diagnostics
Immunological Approaches To Detect Protein Biomarkers
DNA‐Based Diagnostic Approaches
Detecting RNA Signatures of Disease
Biofluorescent and Bioluminescent Systems
Summary
References
Review Questions
5 Protein Therapeutics
Pharmaceuticals
Enzymes
Bacteria and Therapeutics
Recombinant Antibodies
Affibody Molecules
Summary
References
Review Questions
6 Nucleic Acids as Therapeutic Agents
Targeting Specific mRNA and DNA Sequences
Viral Delivery Systems
Nonviral Delivery Systems
Gene Therapy
Summary
References
Review Questions
7 Vaccines
Vaccination
Current and Future Vaccines
Subunit and Peptide Vaccines
Genetic Immunization: DNA Vaccines
Engineered Attenuated Vaccines
Vector Vaccines
Monoclonal Antibody Passive Immunity
Summary
References
Review Questions
8 Industrial and Environmental Uses of Recombinant Microorganisms
Restriction Endonucleases
Small Biological Molecules
Microbial Degradation of Xenobiotics
Utilization of Starch and Sugars
Utilization of Cellulose and Hemicellulose
Lipids from Cyanobacteria
Hydrogen Production
Summary
References
Review Questions
9 Large‐Scale Production of Proteins and Nucleic Acids from Recombinant Microorganisms
Principles of Microbial Growth
Maximizing the Efficiency of the Fermentation Process
Bioreactors
Typical Large‐Scale Fermentation Systems
Harvesting Microbial Cells
Disrupting Microbial Cells
Downstream Processing
Large‐Scale Production of DNA and RNA
Summary
References
Review Questions
10 Genetic Engineering of Plants: Methodology
Plant Transformation with the Ti Plasmid of A. tumefaciens
Ti Plasmid‐Derived Vector Systems
Microprojectile Bombardment
Chloroplast Engineering
Use of Reporter Genes in Transformed Plant Cells
Manipulation of Gene Expression in Plants
Production of Marker‐Free Transgenic Plants
Summary
References
Review Questions
11 Transgenic Plants
Insect Resistance
Virus Resistance
Herbicide Resistance
Fungus and Bacterium Resistance
Salt and Drought Stress
Phytoremediation
Fruits and Flowers
Modification of Plant Nutritional Content
Modification of Food Plant Taste and Appearance
Plants as Bioreactors
Edible Vaccines
Plant Yield
Summary
References
Review Questions
12 Transgenic Animals
Transgenic Animal Methodologies
Transgenic Animal Models of Human Diseases
Animal Bioreactors for Production of Recombinant Therapeutic Proteins
Enhancing Production Traits of Food Animals
Gene Drives To Eradicate Vector‐Transmitted Diseases
Summary
References
Review Questions
13 Molecular Biotechnology and Society
Development of Guidelines for Recombinant DNA Research
Deliberate Release of Genetically Modified Microorganisms
Regulation of Genetically Modified Foods
Societal Concerns about Genetically Modified Foods
Regulation and Safety of Medical Products of Biotechnology
Patenting Biotechnology
Summary
References
Review Questions
Amino Acids of Proteins and Their Designations

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