(Ebook PDF) Textbook of Diagnostic Microbiology 7th Edition by Connie Mahon, Donald Lehman ISBN 9780323832717 0323832717 full chapters

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ISBN 10:0323832717

ISBN 13:9780323832717

Author: Connie R. Mahon; Donald C. Lehman

Gain the knowledge and skills you need to succeed in the clinical lab! Textbook of Diagnostic Microbiology, 7th Edition uses a reader-friendly “building-block” approach to help you learn the essentials of diagnostic microbiology. Featuring full-color drawings and photos, this text helps you learn to develop the critical thinking and problem-solving skills necessary to the accurate diagnosis of infectious diseases and the identification of infectious agents. Written by noted educators Connie R. Mahon and Donald C. Lehman, this edition adds new content on SARS-CoV-2 and COVID-19, along with the latest information on prevention, treatment modalities, and CDC guidelines.

Table of Contents:

• Part 1: Introduction to clinical microbiology
• 1. Bacterial cell structure, physiology, metabolism, and genetics
• Chapter Outline
• Key Terms
• Overview of the microbial world
• Classification/Taxonomy
• Comparison of prokaryotic and eukaryotic cell structure
• Bacterial morphology
• Microbial growth and nutrition
• Bacterial biochemistry and metabolism
• Microbial genetics
• Bibliography
• 2. Host-parasite interaction
• Chapter Outline
• Key Terms
• Origin of microbial biota
• Composition of microbial biota at different body sites
• Role of the microbial biota in the pathogenesis of infectious disease
• Role of the microbial biota in the host defense against infectious disease
• Microbial factors contributing to pathogenesis and virulence
• Host resistance factors
• Mechanisms by which microbes may overcome host adaptive defenses
• Bibliography
• 3. The laboratory role in infection control
• Chapter Outline
• Key Terms
• General concepts in infection prevention and control practice
• Outbreak investigation
• Education
• Response plans
• Bibliography
• 4. Control of microorganisms: disinfection, sterilization, and microbiology safety
• Chapter Outline
• Key Terms
• Disinfection and sterilization
• Sterilization versus disinfection
• Factors that influence the degree of killing
• Methods of disinfection and sterilization
• Disinfectants versus antiseptics
• EPA regulations on chemical surface disinfectants
• FDA regulations on chemical skin antiseptics
• Microbiology laboratory safety
• General laboratory safety
• Bioterrorism and the clinical microbiology laboratory
• Bibliography
• 5. Performance improvement in the microbiology laboratory
• Chapter Outline
• Key Terms
• Introduction to quality management system
• Quality and performance improvement measures
• Quality control in the microbiology laboratory
• Evaluating and interpreting diagnostic laboratory tests
• Analytic analysis of tests
• Clinical analysis of tests
• Operational analysis of tests
• Choosing a laboratory method
• Test validation
• Bibliography
• 6. Specimen collection and processing
• Chapter Outline
• Key Terms
• Basic principles of specimen collection
• Preservation, storage, and transport of specimens
• Specimen receipt and processing
• Culture workup
• Communication of laboratory findings
• Bibliography
• 7. Microscopic examination of materials from infected sites
• Chapter Outline
• Key terms
• Preparation of samples
• Stains
• Microscopes
• Terminology for direct examinations
• Examination of prepared material
• Grading or classifying materials
• Reports of direct examinations
• Initiation of special handling for unsuspected or special pathogens
• Quality control in direct microscopic interpretations
• Examples of sample observations and reports
• Points to remember
• Learning Assessment Questions
• Bibliography
• Direct examination showing local and contaminating materials
• Direct examination in common bacterial infections
• Direct examination in gram-positive bacillary infections
• Direct examination in uncommon gram-positive bacilli
• Direct examination in gram-positive bacilli with filaments and branches
• Direct examination in selected gram-negative bacterial infections
• Direct examination in selected gram-negative bacillary infections
• Direct examination in polymicrobial infections
• 8. Use of colony morphology for the presumptive identification of microorganisms
• Chapter Outline
• Key Terms
• Importance of colony morphology as a diagnostic tool
• Initial observation and interpretation of cultures
• Gross colony characteristics used to differentiate and presumptively identify microorganisms
• Colonies with multiple characteristics
• Growth of microorganisms in liquid media
• Points to Remember
• Learning Assessment Questions
• Bibliography
• 9. Biochemical identification of gram-negative bacteria
• Chapter Outline
• Key terms
• Carbohydrate utilization
• Glucose metabolism and its metabolic products
• Amino acid utilization
• Miscellaneous tests
• Manual multitest systems
• Rapid and automated identification systems
• Points to remember
• Learning assessment questions
• Bibliography
• 10. Immunodiagnosis of infectious diseases
• Chapter Outline
• Key terms
• Antibodies in serologic testing
• Antigen detection
• Principles of immunologic assays
• Use of serologic testing in specific diseases
• Direct antigen detection assays
• Points to remember
• Learning assessment questions
• Bibliography
• 11. Applications of molecular diagnostics
• Chapter Outline
• Key terms
• Nucleic acid hybridization techniques
• Nucleic acid amplification procedures
• Strain typing and identification
• Molecular diagnostics testing in the clinical microbiology laboratory
• Nanomedicine
• Points to Remember
• Learning Assessment Questions
• Bibliography
• 12. Antibacterial mechanisms of action and bacterial resistance mechanisms
• Chapter Outline
• Key Terms
• Antimicrobial targets and mechanisms of action
• Mechanisms of antimicrobial resistance
• β-Lactamase inhibitors
• Dissemination of resistance determinants
• Nanotechnology to deliver therapeutic agents
• Points to remember
• Learning assessment questions
• Bibliography
• 13. Antimicrobial susceptibility testing
• Chapter Outline
• Key Terms
• Procedures in antimicrobial susceptibility testing
• Reasons and indications for performing antimicrobial susceptibility tests
• Selecting antimicrobial agents for testing and reporting
• Traditional antimicrobial susceptibility test methods
• Interpretation of in vitro antimicrobial susceptibility test results
• Automated antimicrobial susceptibility test methods
• Quality control of antimicrobial susceptibility tests
• Selecting an antimicrobial susceptibility test method
• Special antimicrobial susceptibility tests
• Minimum bactericidal concentration test
• Time-kill assays
• Synergy tests
• Serum bactericidal test
• Measurement of antimicrobial agents in serum and body fluids
• Points to remember
• Learning assessment questions
• Bibliography
• Part 2: Laboratory identification of significant isolates
• 14. Staphylococcus and similar organisms
• Chapter Outline
• Key Terms
• General characteristics
• Clinically significant species
• Laboratory diagnosis
• Antimicrobial susceptibility
• Points To Remember
• Learning Assessment Questions
• Bibliography
• 15. Streptococcus, Enterococcus, and other catalase-negative, gram-positive cocci
• Chapter Outline
• Key Terms
• General characteristics
• Clinically significant streptococci and Streptococcus-like organisms
• Laboratory diagnosis
• Points to remember
• Bibliography
• 16. Aerobic gram-positive bacilli
• Chapter Outline
• Key Terms
• Non–spore-forming, nonbranching, catalase-positive bacilli
• Non–spore-forming, nonbranching, catalase-negative bacilli
• Non–spore-forming, branching, aerobic actinomycetes
• Spore-forming, nonbranching, catalase-positive bacilli
• Points to remember
• Learning assessment questions
• Bibliography
• 17. Neisseria species and Moraxella catarrhalis
• Chapter Outline
• Key Terms
• General characteristics
• Pathogenic Neisseria species
• Commensal Neisseria species
• Points to remember
• Learning assessment questions
• Bibliography
• 18. Haemophilus, HACEK group, Legionella, and other fastidious gram-negative bacilli
• Chapter Outline
• Key Terms
• Haemophilus
• Haemophilus influenzae
• HACEK group
• Capnocytophaga
• Pasteurella
• Brucella
• Francisella
• Legionella
• Bordetella
• Bibliography
• 19. Enterobacterales
• Chapter Outline
• Key Terms
• General characteristics
• Opportunistic members of the order Enterobacterales and associated infections
• Primary pathogens in the order Enterobacterales
• Other genera of the order Enterobacterales
• Laboratory diagnosis of the Enterobacterales
• Learning Assessment Questions
• Bibliography
• 20. Vibrio, Aeromonas, Campylobacter, and Campylobacter-like species
• Chapter Outline
• Key Terms
• Vibrio
• Aeromonas
• Campylobacter and Campylobacter-like species
• Learning assessment questions
• Bibliography
• 21. Nonfermenting and miscellaneous gram-negative bacilli
• Chapter Outline
• Key Terms
• General characteristics of nonfermenters
• Clinically significant nonfermentative, gram-negative bacilli
• Less commonly encountered nonfermentative, gram-negative bacilli
• Bibliography
• 22. Anaerobes of clinical importance
• Chapter Outline
• Key Terms
• Important concepts in anaerobic bacteriology
• Frequently encountered anaerobes and their associated diseases
• Specimen selection, collection, transport, and processing
• Procedures for identifying anaerobic isolates
• Antimicrobial susceptibility testing
• Treatment of anaerobe-associated diseases
• Learning assessment questions
• Bibliography
• 23. The spirochetes
• Chapter Outline
• Key terms
• Leptospira
• Treponemes
• Learning assessment questions
• Bibliography
• 24. Chlamydia, Rickettsia, and similar organisms
• Chapter Outline
• Key Terms
• Chlamydiaceae
• Rickettsiaceae and similar organisms
• Points to Remember
• Learning Assessment Questions
• Bibliography
• 25. Mycoplasma and Ureaplasma
• Chapter Outline
• KEY TERMS
• General characteristics
• Clinical infections
• Laboratory diagnosis
• Antimicrobial susceptibility
• Interpretation of laboratory results
• Points to Remember
• Learning Assessment Questions
• Bibliography
• 26. Mycobacterium tuberculosis and nontuberculous mycobacteria
• Chapter Outline
• Key Terms
• General characteristics
• Clinical significance of the Mycobacterium tuberculosis complex
• Clinical significance of nontuberculous mycobacteria
• Mycobacterium leprae
• Isolation and identification of mycobacteria
• Susceptibility testing of Mycobacterium tuberculosis
• Points to Remember
• Learning Assessment Questions
• Bibliography
• 27. Medically significant fungi
• Chapter Outline
• Key Terms
• General characteristics
• Taxonomy
• Mycoses
• Clinically significant species
• Laboratory diagnosis of fungi
• Immunodiagnosis of fungal disease
• Antifungal susceptibility
• Points to Remember
• Learning assessment questions
• Bibliography
• 28. Diagnostic parasitology
• Chapter Outline
• Key Terms
• General concepts in parasitology laboratory methods
• Medically important parasitic agents
• Bibliography
• 29. Clinical virology
• Chapter Outline
• Key Terms
• Characteristics of viruses
• Laboratory diagnosis of viral infections
• Double-stranded DNA viruses
• Single-stranded DNA viruses
• Double-stranded RNA viruses
• Single-stranded RNA viruses
• Hepatitis viruses
• Prions
• Treatment and management of viral infections
• Bibliography
• 30. Agents of bioterror and forensic microbiology
• Chapter Outline
• Key Terms
• Bioterrorism readiness and the clinical laboratory
• Laboratory response network
• General characteristics of biological threat agents
• Biosafety
• Biosafety levels
• Agents of bioterror
• Other biological agents and toxins
• Pandemic preparedness and bioterrorism readiness
• Concluding thoughts
• Forensic microbiology
• Bibliography
• 31. Biofilms: architects of disease
• Chapter Outline
• Key Terms
• Microbial biofilms defined
• Architecture of biofilms
• Biofilm properties
• Mechanisms of pathogenicity
• Diseases associated with biofilms
• Laboratory consequences associated with biofilms
• Detection of biofilms and in vitro formation
• Potential interventions
• Points to Remember
• Learning Assessment Questions
• Bibliography
• Part 3: Laboratory diagnosis of infectious diseases: an organ system approach to diagnostic microbiology
• 32. Upper and lower respiratory tract infections
• Chapter Outline
• Key Terms
• General concepts of infectious diseases of the respiratory tract
• Host risk factors
• Virulence factors of pathogenic organisms
• Upper respiratory tract infections
• Lower respiratory tract infections
• Respiratory tract infections in the immunocompromised host
• Bioterrorism and respiratory infections
• Bibliography
• 33. Skin and soft tissue infections
• Chapter Outline
• Key Terms
• Anatomy of the skin
• Localized bacterial and fungal skin infections
• Dermatologic manifestations of systemic bacterial and fungal infections
• Viral infections
• Parasitic infections
• Immune- or toxin-mediated dermatologic manifestations of infectious agents
• Laboratory diagnosis
• Bibliography
• 34. Gastrointestinal infections and food poisoning
• Chapter Outline
• Key Terms
• Anatomic considerations
• Approach to diagnosis of the patient with diarrhea
• Clinical presentation and pathogenic mechanisms of acute diarrhea
• Common viral, bacterial, and parasitic pathogens
• Diarrhea in special circumstances
• Laboratory diagnosis of gastrointestinal pathogens
• Treatment and prevention of diarrhea
• Bibliography
• 35. Infections of the central nervous system
• Chapter Outline
• Key Terms
• General concepts related to infections of the central nervous system
• Central nervous system infections
• Laboratory diagnosis
• Bibliography
• 36. Bacteremia and sepsis
• Chapter Outline
• Key Terms
• General concepts related to bacteremic infections
• Epidemiology
• Causes
• Pathogenesis
• Clinical aspects of bacteremia
• Laboratory diagnosis
• Treatment
• Prevention
• Bibliography
• 37. Urinary tract infections
• Outline
• Key Terms
• Introduction
• Anatomic considerations
• Epidemiology and risk factors
• Clinical signs and symptoms
• Causes of urinary tract infections
• Laboratory diagnosis
• Microbial detection
• Interpretation of results
• Susceptibility reporting
• Bibliography
• 38. Genital infections and sexually transmitted infections
• Chapter Outline
• Key Terms
• Urethritis
• Cervicitis
• Vulvovaginitis
• Genital ulcer disease
• Acquired immunodeficiency syndrome
• Other sexually transmitted diseases
• Points to Remember
• Bibliography
• 39. Infections in special populations
• Chapter Outline
• Key Terms
• Healthcare-associated infections
• Ventilator-associated pneumonia
• Malignancy
• Acquired immunodeficiency syndrome
• Complement deficiency
• Burns
• Organ transplantation
• Postsplenectomy sepsis
• Cystic fibrosis
• Diabetes
• Aging
• Pregnancy, the fetus, and the neonate
• Bibliography
• 40. Zoonotic diseases
• Chapter Outline
• Key Terms
• Transmission by scratches and bites from domestic or wild animals
• Transmission by direct contact or inhalation
• Transmission by arthropod vectors
• Viral zoonotic diseases
• Emerging zoonoses
• Bibliography
• 41. Ocular infections
• Chapter Outline
• Key Terms
• General concepts related to ocular infections
• Infections of the conjunctivae
• Infections of the eyelids
• Infections of the cornea
• Infections of the sclera and episclera
• Infections of the orbit
• Infections of the lacrimal apparatus
• Infections of the intraocular chambers
• Retinitis
• Biofilm-centered ocular infections
• Laboratory diagnosis of ocular infections
• Bibliography
• Appendix A. Answers to learning assessment questions

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Connie Mahon,Donald Lehman,Diagnostic Microbiology